Gangliosides are versatile molecules fulfilling numerous biological functions and their presence is crucial for the nervous system. The characterization of gangliosides by HPLC-MS enables the determination of the various head and tail group compositions. Employing HILIC (hydrophilic interaction liquid chromatography) results in the separation of gangliosides by their head group, whereas reversed-phase chromatography leads to differentiation according to the tail group composition.

This work was carried out in the group of Prof. Stefan Schürch.

Reference:

• T. Muggli; Master Thesis (2019)